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zeiss inverted fluorescence microscope  (Carl Zeiss)


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    Carl Zeiss zeiss inverted fluorescence microscope
    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron <t>microscope</t> images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead <t>fluorescence</t> images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Zeiss Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 982 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inverted+fluorescent+zeiss+microscope/Inverted+microscope+Axio+Observer+7/pmc13014975-149-14-18
    Average 98 stars, based on 982 article reviews
    zeiss inverted fluorescence microscope - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions"

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2026.103015

    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture

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    Article Snippet: The 96-well plate was subsequently transferred to a Cellomics KineticScan KSR machine. .. The KSR contains a humidified incubator, an inverted fluorescent Zeiss microscope with a high resolution CCD camera, and an integrated computer system. ..

    Article Title: Haptoglobin genotype determines myocardial infarct size in diabetic mice.
    Article Snippet: .. The LV was cut into 15- m-thick cryosections, and very 20th section was photographed using an inverted uorescent Zeiss microscope, connected to a digital camera nd a computer with quantitative ImagePro software (Silver pring, Maryland) (a total of 12 sections for each heart). p he area at risk of MI upon AD ligation was defined and easured as thioflavin negative i.e., the non-blue stained area). he infarct area was defined as ropidium positive regions (i.e., eep red). .. Quantitation of infarct size nd risk area was performed usng an infarct analysis program ith Matlab software, using pixel olor coordinates (color intenity) for automated calculation of he ratios: infarct area/risk area IA/RA), infarct area/left ventrile (IA/LV), risk area/left ventrile (RA/LV).

    Article Title: The DNA damage response (DDR) is induced by the C9orf72 repeat expansion in amyotrophic lateral sclerosis.
    Article Snippet: Briefly, post-mortem spinal cords were blocked with normal goat or horse serum for 30 min and then stained with anti-SMI32 (1:50, Abcam, ab28029), Abcam), anti-VCHAT (1:200, Chemicon, Ab 1578), anti-γ-H2AX (pSer139), 1:100, Calbiochem, DR1017), or anti p-ATM (S1981) antibodies (1:100, Abcam, ab36810) for 48 h at 4◦C, and after three washes with PBS, the tissues were incubated with AlexaFluor 488/564 (1:200, Molecular Probes) conjugated secondary antibodies. .. Images were acquired using an inverted fluorescent Zeiss microscope (Zeiss Instruments, Jena, Germany). ..

    Article Title: The DNA damage response (DDR) is induced by the C9orf72 repeat expansion in amyotrophic lateral sclerosis.
    Article Snippet: Cells were transfected transiently with empty CMV vector only or with poly(GR)100 and poly(PR)100 (500ng DNA per well) using lipofectamine 2000 (Invitrogen/ LifeTechnologies), according to the manufacturer’s instructions. .. After 72 hours cells were examined using an inverted fluorescent Zeiss microscope (Zeiss Instruments, Jena, Germany). .. Nucleolar stress and NPM1 distribution SH-SY5Y human neuroblastoma cells were transfected with empty CMV vector only or with poly(GR)100 and poly(PR)100 using lipofectamine 2000, and immunocytochemistry using anti- NPM1 antibodies was performed to examine the distribution of NPM1; nuclei were stained with Hoechst 33342 (Sigma-Aldrich).

    Article Title: Nitric oxide receptor soluble guanylyl cyclase undergoes splicing regulation in differentiating human embryonic cells.
    Article Snippet: .. Digital images were acquired with an inverted fluorescent Zeiss microscope and Zeiss image capturing software. .. For confocal microscopy, cells were detached by treatment with 2 mg=mL collagenase IV (Invitrogen), sedimented on a slide by centrifugation, and processed as described above, except that ToPro-3 dye was used for nuclei staining.

    Software:

    Article Title: Haptoglobin genotype determines myocardial infarct size in diabetic mice.
    Article Snippet: .. The LV was cut into 15- m-thick cryosections, and very 20th section was photographed using an inverted uorescent Zeiss microscope, connected to a digital camera nd a computer with quantitative ImagePro software (Silver pring, Maryland) (a total of 12 sections for each heart). p he area at risk of MI upon AD ligation was defined and easured as thioflavin negative i.e., the non-blue stained area). he infarct area was defined as ropidium positive regions (i.e., eep red). .. Quantitation of infarct size nd risk area was performed usng an infarct analysis program ith Matlab software, using pixel olor coordinates (color intenity) for automated calculation of he ratios: infarct area/risk area IA/RA), infarct area/left ventrile (IA/LV), risk area/left ventrile (RA/LV).

    Article Title: Nitric oxide receptor soluble guanylyl cyclase undergoes splicing regulation in differentiating human embryonic cells.
    Article Snippet: .. Digital images were acquired with an inverted fluorescent Zeiss microscope and Zeiss image capturing software. .. For confocal microscopy, cells were detached by treatment with 2 mg=mL collagenase IV (Invitrogen), sedimented on a slide by centrifugation, and processed as described above, except that ToPro-3 dye was used for nuclei staining.

    Ligation:

    Article Title: Haptoglobin genotype determines myocardial infarct size in diabetic mice.
    Article Snippet: .. The LV was cut into 15- m-thick cryosections, and very 20th section was photographed using an inverted uorescent Zeiss microscope, connected to a digital camera nd a computer with quantitative ImagePro software (Silver pring, Maryland) (a total of 12 sections for each heart). p he area at risk of MI upon AD ligation was defined and easured as thioflavin negative i.e., the non-blue stained area). he infarct area was defined as ropidium positive regions (i.e., eep red). .. Quantitation of infarct size nd risk area was performed usng an infarct analysis program ith Matlab software, using pixel olor coordinates (color intenity) for automated calculation of he ratios: infarct area/risk area IA/RA), infarct area/left ventrile (IA/LV), risk area/left ventrile (RA/LV).

    Staining:

    Article Title: Haptoglobin genotype determines myocardial infarct size in diabetic mice.
    Article Snippet: .. The LV was cut into 15- m-thick cryosections, and very 20th section was photographed using an inverted uorescent Zeiss microscope, connected to a digital camera nd a computer with quantitative ImagePro software (Silver pring, Maryland) (a total of 12 sections for each heart). p he area at risk of MI upon AD ligation was defined and easured as thioflavin negative i.e., the non-blue stained area). he infarct area was defined as ropidium positive regions (i.e., eep red). .. Quantitation of infarct size nd risk area was performed usng an infarct analysis program ith Matlab software, using pixel olor coordinates (color intenity) for automated calculation of he ratios: infarct area/risk area IA/RA), infarct area/left ventrile (IA/LV), risk area/left ventrile (RA/LV).



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    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    doi: 10.1016/j.mtbio.2026.103015

    Figure Lengend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Following the 30 - min incubation in the dark, images were collected with the ZEISS inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany; Model: Axio Observer 7).

    Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture